 | Kevin James als Otis the Cow (voice) |
 | Courteney Cox als Daisy the Cow (voice) |
 | Sam Elliott als Ben the Cow (voice) |
 | Danny Glover als Miles the Mule (voice) |
 | Wanda Sykes als Bessy the Cow (voice) |
 | Andie MacDowell als Etta the Hen (voice) |
 | David Koechner als Dag the Coyote (voice) |
 | Jeffrey Garcia als Pip the Mouse (voice) |
 | Cam Clarke als Freddy the Ferret (voice) |
 | Rob Paulsen als Peck the Rooster / Gopher / Pizza Twin #1(voice) |
 | Tino Insana als Pig the Pig (voice) |
 | Dom Irrera als Duke the Dog (voice) |
 | S. Scott Bullock als Eddy the Cow (voice) |
 | John DiMaggio als Bud the Cow / Officer O'Hanlon (voice) |
 | Maurice LaMarche als Igg the Cow (voice) |
| Madeline Lovejoy als Maddy the Chick (voice) |
 | Earthquake als Root the Rooster (voice) |
 | Steve Oedekerk als Snotty Boy / Mr. Beady / Snotty Boy's Father (voice) |
 | Maria Bamford als Mrs. Beady (voice) |
 | Fred Tatasciore als Farmer (voice) |
| Peter Stenstrom als Skunk (voice) |
 | Jill Talley als Snotty Boy's Mother (voice) |
 | Laraine Newman als Snotty Boy's Friend (voice) |
 | Katie Leigh als Snotty Boy's Friend / Additional Barnyard Voices (voice) |
| North Mississippi Allstars als Barn Boys (voice) |
| Anna Pistor als Chick (voice) |
| Thomas Pistor als Chick (voice) |
| Eliana Bendetson als Chick (voice) |
 | Paul Butcher als Chick (voice) |
 | Khamani Griffin als Chick (voice) |
| Arlo Levin als Chick (voice) |
 | Liliana Mumy als Chick (voice) |
| Cydney Neal als Chick (voice) |
| Cate Ozawa als Chick (voice) |
| Isaiah Tefilo als Chick (voice) |
| Georgia Van Newkirk als Chick (voice) |
| Joni Allen als Singer (voice) |
 | Leigh French als Additional Barnyard Voices (voice) |
| William Calvert als Additional Barnyard Voices (voice) |
 | Julianne Buescher als Additional Barnyard Voices (voice) |
 | Nicholas Guest als Additional Barnyard Voices (voice) |
 | Audrey Wasilewski als Additional Barnyard Voices (voice) |
 | Phil Proctor als Additional Barnyard Voices (voice) |
 | Archie Hahn als Additional Barnyard Voices (voice) |
 | Chad Einbinder als Additional Barnyard Voices (voice) |
| Keith Anthony als Additional Barnyard Voices (voice) |
| Claudette Wells als Additional Barnyard Voices (voice) |
 | Lynne Marie Stewart als Additional Barnyard Voices (voice) |
| Christie Mellor als Additional Barnyard Voices (voice) |
 | Justin Shenkarow als Additional Barnyard Voices (voice) |
 | Jacqueline Pinol als Additional Barnyard Voices (voice) |
 | Nika Futterman als Additional Barnyard Voices (voice) |
 | Eddie Frierson als Additional Barnyard Voices (voice) |
| Paul Marshall | Producer |
| Philip A. Cruden | Art Direction |
| John Debney | Original Music Composer |
| Steve Oedekerk | Producer |
| Julia Pistor | Executive Producer |
| Paul D. Calder | Editor |
| Philip A. Cruden | Production Design |
| Steve Oedekerk | Director |
| Aaron Parry | Executive Producer |
| Andre Medina | Animation |
| Eric Oliver | Animation |
| Leonard F.W. Green | Special Effects |
| Bill Schwab | Art Department Manager |
| Rick Moser | Compositors |
| Ivaylo Anguelov | Layout |
| Kevin Kaska | Orchestrator |
| Billy Weber | Editor |
| Mark Beam | Associate Producer |
| Nicholisa Contis | Associate Producer |
| Andrew Egiziano | Associate Producer |
| A.K. Madhavan | Executive Producer |
| Pam Marsden | Producer |
| Curtis A. Koller | Casting |
| Leslie Barker | Production Manager |
| Ramsey Ann Naito | Executive In Charge Of Production |
| Glenn Curry | Storyboard Artist |
| Asako Miyamori | Set Dresser |
| Kyle Morgan | Art Department Production Assistant |
| Odin Benitez | Sound Designer |
| Devon Bowman | Production Sound Mixer |
| G.W. Brown | ADR Editor |
| Yann Delpuech | Sound Effects Editor |
| Jeff Gomillion | ADR Mixer |
| Gabriel Guy | Sound Mix Technician |
| Robin Harlan | Foley Artist |
| Michael Hilkene | Supervising Sound Editor |
| William Jacobs | Sound Editor |
| Linda Lew | Foley Editor |
| Ryan Maguire | Foley Mixer |
| Anand Pawar | Dialogue Editor |
| Philip Rogers | ADR Recordist |
| Pernell L. Salinas | First Assistant Sound Editor |
| James Wright | Dolby Consultant |
| Steve Baker | Animation Supervisor |
| Patrick Beauchamp | Lighting Artist |
| Tom Capizzi | CG Supervisor |
| Rosie Galvin | Visual Effects Coordinator |
| Annick Harmel-Tourneur | Rigging Supervisor |
| Steve Kolbe | Layout Supervisor |
| Martin Lauzon | Visual Effects Supervisor |
| Joaquin Pecheur | Digital Compositor |
| Johann Quéré | Visual Effects |
| Bharat Waskar | Matte Painter |
| Charlie Winter | Lighting Supervisor |
| Mel Rapster | Lighting Production Assistant |
| Mark Beam | Character Designer |
| Mike Bodkin | Production Coordinator |
| Steven Clay Hunter | Supervising Animator |
| Dominique Monfery | Animation Director |
| Zachary J. Rose | Animation Production Assistant |
| Mark Dinicola | Digital Intermediate Colorist |
| Christine Dougherty | Digital Intermediate Producer |
| Dale E. Grahn | Color Timer |
| Margaret Johnson-Holzendorf | Associate Editor |
| Danik Thomas | Assistant Editor |
| Matt Voges | Editorial Coordinator |
| Mark Berrow | Musician |
| Jeff Carson | Music Editor |
| John Debney | Conductor |
| Brad Dechter | Orchestrator |
| Leo Josefsson | Music Producer |
| Karyn Rachtman | Executive Music Producer |
| Angela Reid | Music Coordinator |
| Kristine Familletti | Script Supervisor |
| Steven C. Butwell | Production Assistant |
| Sydney S Matthews | Production Intern |
| Sonia Meza-Leon | First Assistant Accountant |
| Todd Grimes | Character Modelling Supervisor |
| Steve Oedekerk | Writer |
