 | Tom Holland als Peter Parker / Spider-Man |
 | Zendaya als Michelle 'MJ' Jones |
 | Benedict Cumberbatch als Stephen Strange / Doctor Strange |
 | Jacob Batalon als Ned Leeds |
 | Jon Favreau als Harold 'Happy' Hogan |
 | Jamie Foxx als Max Dillon / Electro |
 | Willem Dafoe als Norman Osborn / Green Goblin |
 | Alfred Molina als Dr. Otto Octavius / Doctor Octopus |
 | Benedict Wong als Wong |
 | Tony Revolori als Eugene 'Flash' Thompson |
 | Marisa Tomei als May Parker |
 | Andrew Garfield als Peter Parker / Spider-Man |
 | Tobey Maguire als Peter Parker / Spider-Man |
 | Angourie Rice als Betty Brant |
 | Arian Moayed als Agent Cleary |
 | Paula Newsome als MIT Assistant Vice Chancellor |
 | Hannibal Buress als Coach Wilson |
 | Martin Starr als Mr. Harrington |
 | J.B. Smoove als Mr. Dell |
 | J.K. Simmons als J. Jonah Jameson |
 | Rhys Ifans als Dr. Curt Connors / The Lizard |
 | Charlie Cox als Matt Murdock |
 | Thomas Haden Church als Flint Marko / Sandman |
 | Emily Fong als Apprentice |
 | Mary Rivera als Ned's Lola |
 | Rudy Eisenzopf als Arena Bystander |
 | Kathleen Cardoso als Arena Bystander |
 | Jonathan Sam als Arena Bystander |
| Andrew Dunlap als Dad in Car |
 | Ben VanderMey als Man in Car |
 | Zany Dunlap als Mom in Car |
 | B. Clutch Dunlap als Kid in Car |
 | Minnah Dunlap als Kid in Car |
 | Gary Weeks als Agent Foster |
 | Gregory Konow als Donut Shop Boss |
 | Carol Anne Dines als Donut Shop Waitress |
 | Anisa Nyell Johnson als School Security Guard |
| Willie Burton als May's Neighbor |
 | Mallory Hoff als Reporter |
 | Greg Clarkson als Reporter |
 | Regina Ting Chen als Reporter |
 | Robert Mitchel Owenby als Daily Bugle Lackey |
 | Glenn Keogh als British Reporter |
 | Paris Benjamin als British Anchor |
| Jwaundace Candece als Damage Control Agent |
 | Taylor St. Clair als Protestor |
 | Gabriella Cila als Crowd Member |
| Darnell Appling als Crowd Member |
 | Rolando Fernandez als Crowd Member |
 | Edward Force als Donut Shop Patron |
| Michael Le als Guy with Phone |
 | Dean Meminger als NY1 Reporter |
| Frederick A. Brown als Fred the Janitor |
 | Cristo Fernández als Bartender |
 | Clay Savage als Suit Voice (voice) |
 | Tom Hardy als Eddie Brock / Venom (uncredited) |
 | Jake Gyllenhaal als Mysterio / Quentin Beck (archive footage) (uncredited) |
 | Jay Karales als Bodega Customer (uncredited) |
 | Gina Aponte als Media Reporter (uncredited) |
| John Barnes als School Protester (uncredited) |
 | Harry Holland als Shaker Kid (uncredited) |
 | Haroon Khan als Apprentice |
| John M. Maiers als Paparazzi |
| Amy Pascal | Producer |
| Kevin Feige | Producer |
| Jon Watts | Director |
| Stan Lee | Characters |
| Darren Gilford | Production Design |
| Sarah Halley Finn | Casting |
| Erik Sommers | Writer |
| Chris McKenna | Writer |
| Steve Ditko | Characters |
| Michael Giacchino | Original Music Composer |
| Linda D. Flowers | Hair Department Head |
| Vasilios Tanis | Makeup Department Head |
| Steven S. Shapiro | Production Executive |
| George Cottle | Second Unit Director |
| Brian Avery Galligan | Second Unit First Assistant Director |
| David H. Venghaus Jr. | First Assistant Director |
| Rosemary Brandenburg | Set Decoration |
| Emmanuelle Hoessly | Set Decoration |
| Richard Bennett | Storyboard Artist |
| Russell Bobbitt | Property Master |
| Andrew Campbell | Graphic Designer |
| Nick S. Cross | Set Designer |
| George Cottle | Stunt Coordinator |
| Molly Doyle | Casting Associate |
| Louis D'Esposito | Executive Producer |
| Victoria Alonso | Executive Producer |
| JoAnn Perritano | Executive Producer |
| Rachel O'Connor | Executive Producer |
| Avi Arad | Executive Producer |
| Matt Tolmach | Executive Producer |
| Samantha Avila | Art Direction |
| Frances Lynn Hernandez | Art Direction |
| Kristen Maloney | Art Direction |
| Brian Stultz | Art Direction |
| Heather Benson | Makeup Artist |
| Nancy Cummings | Makeup Artist |
| Yvonne Eagle | Makeup Artist |
| Carla Wallace | Makeup Artist |
| Chiranjiv Makwana | Animation Director |
| Mauro Fiore | Director of Photography |
| Eric Mancha | VFX Artist |
| David H. Venghaus Jr. | Associate Producer |
| Sheika Daley | Makeup Artist |
| Shawn Janifer | Makeup Artist |
| Darla Wigley | Makeup Artist |
| Patrick Dunn-Baker | Set Designer |
| Sally Ledger | Assistant Art Director |
| Kristen Jenkins | Set Designer |
| George Lee | Set Designer |
| Tammy S. Lee | Set Designer |
| Silvia Mahapatra | Set Designer |
| Nicanor Mendoza III | Set Designer |
| Anne Porter | Set Designer |
| Chris Sanford | Set Designer |
| Justin Trudeau | Set Designer |
| Justin M. Davey | Sound Effects Editor |
| Sam Fan | Sound Effects Editor |
| Jamie Hardt | Sound Effects Editor |
| Takako Ishikawa | Sound Effects Editor |
| Tony Lamberti | Sound Re-Recording Mixer |
| Ken McGill | Sound Designer |
| Kevin O'Connell | Sound Re-Recording Mixer |
| Steven Ticknor | Supervising Sound Editor |
| Ed Bruce | Visual Effects Supervisor |
| Diana Cobo | Visual Effects Producer |
| Scott Edelstein | Visual Effects Supervisor |
| Bryan Haines | VFX Artist |
| Daniel Hazeltine | VFX Artist |
| Michael Killoren | Visual Effects Producer |
| Nicholas Murphy | Visual Effects Producer |
| Brendan Seals | Visual Effects Supervisor |
| Cody Stoof | VFX Artist |
| Chris Waegner | Visual Effects Supervisor |
| Kate Abraham | Set Costumer |
| Kevin Boyett | Set Costumer |
| David Butler | Set Costumer |
| Jessica Pazdernik | Costume Supervisor |
| Nava R. Sadan | Costume Supervisor |
| Addy Steigerwalt | Set Costumer |
| Pamela Waggoner | Set Costumer |
| Lahiru Jay | VFX Artist |
| Kelly Port | Visual Effects Supervisor |
| Dave Jordan | Music Supervisor |
| Stan Lee | Comic Book |
| Steve Ditko | Comic Book |
| Greg Townley | Acting Double |
| Luke Scott | Acting Double |
| Martin De Boer | Acting Double |
| Lloyd Pitts | Acting Double |
| William R. Spencer | Stunt Double |
| David Elson | Stunt Double |
| Sergio Garcia | Stunt Double |
| Ben Jenkin | Stunt Double |
| Jeffrey Ford | Editor |
| Leigh Folsom Boyd | Editor |
| Sanja Milković Hays | Costume Design |
| Satoshi Takazawa | VFX Artist |
| David Scott | Supervising Art Director |
| Clint Wallace | Additional Photography |
| Ali Safdari | Art Direction |
| Ellen Arden | Key Makeup Artist |
| Kat Connelly | Key Makeup Artist |
| Bridgit Crider | Key Makeup Artist |
| Lane Friedman | Hair Department Head |
| Lane Friedman | Key Hair Stylist |
| Donald McInnes | Hairstylist |
| Donald McInnes | Makeup Artist |
| Dominic Mombrun | Prosthetics |
| Anne Morgan | Hairstylist |
| Tim Muir | Hairstylist |
| Shuayb Naimo | Makeup Artist |
| Yeşim "Shimmy" Osman | Hairstylist |
| Lali Peer | Makeup Artist |
| Lali Peer | Additional Photography |
| Rachael Speke | Hairstylist |
| Rachael Speke | Makeup Artist |
| Emily Fong | Associate Producer |
| Gary Archer | Prosthetics |
| Brian Michael Bendis | Thanks |
| Robert Bernstein | Thanks |
| Giuseppe Camuncoli | Thanks |
| Olivier Coipel | Thanks |
| Ernie Colon | Thanks |
| Bill Everett | Thanks |
| Don Heck | Thanks |
| Dwayne McDuffie | Thanks |
| Sara Pichelli | Thanks |
| Joe Quesada | Thanks |
| Paolo Rivera | Thanks |
| John Romita Jr. | Thanks |
| Dan Slott | Thanks |
| J. Michael Straczynski | Thanks |
| Reetu Aggarwal | 3D Artist |
| Timothy Eulich | Stunts |
| Jennie Shea | ADR Recordist |
| James Henry Benton | Grip |
| Vinii Khullar | Sound Mixer |
| Marie Fink | Stunts |
| Eric Stratemeier | Stunts |
| Marian Green | Stunts |
| Duffy Gaver | Stunts |
| Robert Smithson | Contact Lens Technician |
