 | Bela Lugosi als Scientist |
 | Edward D. Wood Jr. als Glen / Glenda (as Daniel Davis) |
 | Lyle Talbot als Inspector Warren |
 | Timothy Farrell als Dr. Alton / Narrator |
 | Dolores Fuller als Barbara |
 | 'Tommy' Haynes als Alan / Anne |
| Charlie Crafts als Johnny (as Charles Crafts) |
 | Conrad Brooks als Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks) |
| Henry Bederski als Man with Hat and Receding Hairline (uncredited) |
| Carol Daugherty als Woman in Nightmare (uncredited) |
| 'Captain' DeZita als The Devil / Glen's Father (uncredited) |
| Helen Miles als (uncredited) |
| Shirley Speril als Miss Stevens (uncredited) |
 | Amzie Strickland als Minor Role (uncredited) |
| Harry Thomas als Man in Nightmare (uncredited) |
| William C. Thompson als Judge (uncredited) |
| 'Mr. Walter' Hadjwiecyz als Patrick / Patricia (uncredited) |
| George Weiss als Man at Transvestite's Suicide (uncredited) |
| Evelyn Wood als Sheila, Glen's Sister (uncredited) |
| Edward D. Wood Jr. | Director |
| William C. Thompson | Director of Photography |
| George Weiss | Producer |
| Edward D. Wood Jr. | Story |
| Edward D. Wood Jr. | Screenplay |
| Sanford H. Dickinson | Music Supervisor |
| Ben Winkler | Sound Recordist |
| Bud Schelling | Editor |
| Scott McCloud | Second Unit |
| Harry Thomas | Makeup Artist |
| Jack Miles | Settings |
| Bert Shipman | Camera Operator |
| Larry Smith | Still Photographer |
| Nathan Bailey | Medical Consultant |
